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There are the slides from the conference I just gave during Eurofurence18 last week. It was mostly an update on my previous findings since last year's talk, but also covered new promising avenues about 3D printing of whole parts of body (tail, muzzle...) and animal uplifting through testis-based genetic engineering.
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Category Story / Animal related (non-anthro)
Species Unspecified / Any
Size 76 x 120px
File Size 1.96 MB
Listed in Folders
Very thorough and well put together series of presentations, excellent work.
One little relevant note is that it is starting to look like CRISPER and the TALEN/TAL effectors might be better then the zinc finger nucleases for all the knock-out, knock-in, and other related genome editing work. Of course zinc finger nucleases still have their place, especially as the Aldrich SAGE laboratories has a catalog that sells specially designed zinc finger nucleases (ZFN) to target every single gene in the human and mouse genomes individually as desired. But still, if you want to do custom editing at DNA loci for which ZFN are not available for purchase directly from Aldrich, CRISPER and TALEN/TAL would be much cheaper, faster, and some signs point to it being a little more accurate as well in terms of less star activity and other such errors.
One little relevant note is that it is starting to look like CRISPER and the TALEN/TAL effectors might be better then the zinc finger nucleases for all the knock-out, knock-in, and other related genome editing work. Of course zinc finger nucleases still have their place, especially as the Aldrich SAGE laboratories has a catalog that sells specially designed zinc finger nucleases (ZFN) to target every single gene in the human and mouse genomes individually as desired. But still, if you want to do custom editing at DNA loci for which ZFN are not available for purchase directly from Aldrich, CRISPER and TALEN/TAL would be much cheaper, faster, and some signs point to it being a little more accurate as well in terms of less star activity and other such errors.
I learned about the TALEN effectors just before crafting this talk, so i decided not to include them this time.
The current strategy that I can envision would be to realize a Testis-Mediated Gene Transfer 1) using bacteria modified to penetrate mammalian cells 2) and loaded with a compatible artificial chromosome 3) containing both the genes to add and RNA interferons to silence the genes to replace. TMGT and its sperm equivalent is simpler and much more efficient than somatic cell transfer (50-80% success rate against 2-5%) and allows to use the tools that you mentioned for a precise insertion into the target genome.
1) http://2012.igem.org/Team:Warsaw
2) http://www.nature.com/gt/journal/v1.....t2010147a.html
3) http://2009.igem.org/Team:Bologna
The current strategy that I can envision would be to realize a Testis-Mediated Gene Transfer 1) using bacteria modified to penetrate mammalian cells 2) and loaded with a compatible artificial chromosome 3) containing both the genes to add and RNA interferons to silence the genes to replace. TMGT and its sperm equivalent is simpler and much more efficient than somatic cell transfer (50-80% success rate against 2-5%) and allows to use the tools that you mentioned for a precise insertion into the target genome.
1) http://2012.igem.org/Team:Warsaw
2) http://www.nature.com/gt/journal/v1.....t2010147a.html
3) http://2009.igem.org/Team:Bologna
Found them again, thank goodness I saved some as favourites. https://www.youtube.com/watch?v=iFG3zQc5RSo
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