Femshep, Tali, Lydia and Leliana to name a few

My favorite girl from a video game would have to be KOS-MOS from Xenosaga. Here's a good example of her with my oc http://www.furaffinity.net/view/16097524/
Zero from Drakenguard 3 is also my favorite, along with Lenneth from Valkyrie Profile Paulitena from Kid Icarus and Lucina from Fire Emblem

female character from saints row 3

Does GLaDOS count?

Not sure... To many good looking girl outhere!

It can be Sylvanas from WoW, Fran from Final Fantasy XII, Taky or Ivy from Soul Calibur (i prefer them in the III insted the IV... But you know... That kind of games have super breasty girl XD), Jinx from LoL... Then i don't remind any girl who really impress me as looking / story

fuck, that's a hard question so early in the morning there, hun. :/

I do really like Sophie from Tales of Graces.
I guess Zelda and Malon from OoT circa Loz games are sorta crushes.
Ayla from Chrono Trigger was really bad-ass! even though I wish she could get officially rendered in another style XD

I really should also toss in Samus for good measure. =3